PCR-diagnosis of Anaplasma marginale in cattle populations of Ecuador and its molecular identification through sequencing of ribosomal 16S fragments

Table 1 Sequence of primers used for amplification of msp5 gene and 16S rRNA

Primer	5′-3′ Sequence	Temp. hybrid	Target	Reference and target
Ana 19A	GTGTTCCTGGGGTACTCCTA	64 °C	MSP5	Reyna-Bello et al., 1998
Ana 19B	TGATCTGGTCAGCCCCAGCT
RYaF16S	TAACACATGCAAGTCGAACGG	68 °C		Chacón 2012
RYaR16S	ACCCAACCTTAAATGGTCGC
M13 F M13 R	ACATTCCAGCAGCAGTTC GAG CACGTGAATCCTCAATTTTGT	63 °C	Fragment inserted into the plasmid	Invitrogen™

ISSN: 1746-6148