Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves

BMC Veterinary Research

Table 4 Sequences of oligonucleotide primers selected as reference genes for qPCR analysis

Gene ID^a	Primers (5′-3′)	Accession No.	Amplicon length
Act- β	Forward: CGGCATCGAGGACAGGAT Reverse: CATCGTACTCCTGCTTGCTGAT	NM_173979.3	169
GAPDH	Forward: CCTGCCCGTTCGACAGATA Reverse: GGCGACGATGTCCACTTTG	NM_001034034.1	150
FAU	Forward: CCGCATGCTTGGAGGTAAAG Reverse: CACAACATTGACAAAGCGCC	NM_174731.3	154
RPS9	Forward: CCGCATGCTTGGAGGTAAAG Reverse: CACAACATTGACAAAGCGCC	NM_001101152.2	188
HPRT1	Forward:GGATTACATCAAAGCACTGAACA Reverse: CATTGTCTTCCCAGTGTCAATT	NM_001034035	194

^a Act- β β-actin, GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase, FAU Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed, RPS9 Ribosomal Protein S9, HPRT1 Hypoxanthine Phosphoribosyltransferase 1

ISSN: 1746-6148