Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves

BMC Veterinary Research

Table 1 Sequences of oligonucleotide primers used for qPCR analysis of lung tissue

Gene ID^a	Primers (5′-3′)	Accession No.	Amplicon length
MUC5AC	Forward: CAGTACAGAGTGCATGGGGA Reverse: TTCACAAACACCTCCCCACT	XM_015470102.1	185
MUC5B	Forward: AAAACGCCCTTCACCTTCAC Reverse: TGCCTCAGGTTCTCGAATGT	XM_015470101.1	176
LAP	Forward: CCCTGGAAGCATGAGACAGA Reverse: TTTCTGACTCCGCATCCAGT	S76279.1	109
SP-A	Forward: TGGGGAGGCATCTTGTTAGG Reverse: TGTTCATCAGCAGGCAGGTA	NM_001077838.2	194
SP-B	Forward: GACATGTGGAAGCCGATGAC Reverse: TGAGTCCTGGAAAATGGCCT	NM_001075311.2	92
SP-C	Forward: GAGATCCAGGAGCAAAGGGT Reverse: CCTCCCACAGTCCCATTTCT	NM_174462.4	95
SP-D	Forward: CCTGTACCCTGGTCATGTGT Reverse: AGCAGAGCCATTGTCTCCTT	NM_181026.2	179
CYP1A1	Forward: GTCACAACTGCCCTTTCCTG Reverse: AAAGGAGGAGTGTCGGAAGG	XM_005192890.3	180
CYP1A2	Forward: TCCTCTTCCTGGCCATCTTG Reverse: CAGAACGCCAGCAACTTCTT	XM_015468527.1	165
ABCA3	Forward: GAGCACACCTTCAACCACAG Reverse: AAAGGAGCCTGTCTGAGTCC	NM_001113746.1	108
SCN11A	Forward: ATGCTGGCTTTAATCTGCGG Reverse: CCTGGAAGCACGAATGGATG	NM_174598.3	177
SCN11B	Forward: CTGAAGGACCTGGACGAACT Reverse: TTGATAAAGACCAGGGGCGT	NM_001098075.1	128
SGK-1	Forward: GGCTCGATTCTATGCTGCTG Reverse: ACGTTGTGCCATTGTGTTCA	NM_001102033.1	121

^a MUC5AC mucin 5 ac, MUC5B mucin 5B, LAP lingual antimicrobial peptide, SP-A Surfactant protein A, SP-B Surfactant protein B, SP-C surfactant protein C, SP-D surfactant protein D, CYP1A1 cytochrome P45 family 1 subfamily A member 1, CYP1A2 cytochrome P450 family 1 subfamily A member 2, ABCA3 ATP binding cassette subfamily A member 3, SCN11A Sodium channel voltage-gated type 2 alpha subunit, SCN11B Sodium channel voltage-gated type 2 beta subunit, SGK-1 Serum/glucocorticoid regulated kinase 1

ISSN: 1746-6148