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Fig. 4 | BMC Veterinary Research

Fig. 4

From: 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle

Fig. 4

MoDCs differentiated in the presence of 1,25(OH)2D3 are not able to induce an allogeneic mixed leukocyte reaction. MoDCs differentiated from cattle CD14 monocytes in the presence or absence of 1,25(OH)2D3 for 10 days. After harvesting, cells incubated with or without LPS for 24 h. Following stimulation, cells were irradiated and cultured in quadruplicate at different ratios with 105responder PBMC for 5 days. Responder PBMC and stimulator MoDCs were incubated in medium only or with ConA (5μg/ml) as controls of proliferation. Proliferation was measured by the incorporation of methyl-3H thymidine ([3H]TdR; 0.5 μCi per well) for the final 18 h of culture. Data are presented as the corrected counts per minute (ccpm) averaged over 3 min. Data shown are the representative of two independent experiments with error bars denoting ± SE. Dashed line denotes the spontaneous proliferation of PBMC with no ConA/stimulator cells. * denotes statistical significance for p < 0.05, ** p value between 0.001 and 0.01 and *** for p < 0.001

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