Fig. 3From: 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattleThe endocytic capabilities of cattle MoDCs are diminished by conditioning with 1,25(OH)2D3. MoDCs were differentiated from cattle CD14 monocytes in the absence (black bars) or presence (white bars) of 1,25(OH)2D3 for 10 days. After harvesting, cells were incubated for 1 h with DQ-OVA and uptake analysed by flow cytometry. Live, single cells were gated and the MFI of each treatment calculated. The results shown are average ± SE of two animals representing the DQ MFI for uptake by MoDCs and 1,25(OH)2D3 conditioned MoDCs. * denotes statistical significance for p < 0.05Back to article page