Fig. 4From: Molecular cloning and characterization of the family of feline leucine-rich glioma-inactivated (LGI) genes, and mutational analysis in familial spontaneous epileptic catsPhylogenetic trees of the LGI family. The phylogenetic trees of the LGI family proteins were constructed to investigate the evolutionary relationships using the neighbor-joining method with Molecular Evolutionary Genetics Analysis Version (MEGA) 7.0 to calculate the p-distance, 1000 bootstrap replication, and pairwise deletion for the gaps/missing data treatment. Amino acid sequences of the LGI family used for this construction were obtained from GenBank, and their accession numbers are shown in Additional file 10. a LGI1 protein, b LGI2 protein, c LGI3 protein, and d LGI4 protein. The numbers at the nodes indicate bootstrap majority consensus values from 1000 replicatesBack to article page