Fig. 9
Mean (±SD) level of antibodies against Haemophilus parasius in of pigs single or dual inoculated with swine influenza virus (SIV) and/or Haemophilus parasius (Hps) * - significant differences compared to control group; a - significant differences between co-inoculated pigs and Hps single-inoculated groups. ELISA ratio (ER) was calculated as directed by the manufacturer (Swinecheck®HPS, Biovet, Canada) according to the following formula: \( \mathrm{Er}=\frac{\mathrm{OD}\ \mathrm{of}\ \mathrm{test}\ \mathrm{serum}\ \mathrm{from}\ \mathrm{wells}\ \mathrm{with}\ \mathrm{Ag}\hbox{-} \mathrm{OD}\ \mathrm{of}\ \mathrm{test}\ \mathrm{serum}\ \mathrm{from}\ \mathrm{wells}\ \mathrm{with}\mathrm{out}\ \mathrm{Ag}}{\mathrm{mean}\ \mathrm{OD}\ \left(+\right)\ \mathrm{from}\ \mathrm{wells}\ \mathrm{with}\ \mathrm{Ag}\hbox{-} \mathrm{mean}\ \mathrm{OD}\ \left(+\right)\ \mathrm{from}\ \mathrm{wells}\ \mathrm{with}\mathrm{out}\ \mathrm{Ag}\ } \) (+) - positive control, Ag - antigen