Characterization and susceptibility of streptococci and enterococci isolated from Nile tilapia (Oreochromis niloticus) showing septicaemia in aquaculture and wild sites in Egypt

Table 1 Oligonucleotide primers sequences and size of the PCR-targeted products

Microorganism	Target Gene	Bp fragment	Primer sequence (5′ - 3′)	Annealing Temp (°C)	References
E. faecalis / E. gallinarum	16S rRNA (Genus -specific primers)	112 bp	F- TAC TGA CAA ACC ATT CAT GAT G	59 (E. faecalis) 50 (E. gallinarum)	[23]
E. faecalis / E. gallinarum	16S rRNA (Genus -specific primers)	112 bp	R- AAC TTC GTC ACC AAC GCG AAC	59 (E. faecalis) 50 (E. gallinarum)	[23]
E. faecalis	ddl _E.fecalis (Species-specific primers)	941 bp	F- ATC AAG TAC AGT TAG TCT	55	[24]
E. faecalis	ddl _E.fecalis (Species-specific primers)	941 bp	R- ACG ATT CAA AGC TAA CTG	55	[24]
L. garvieae	16S rRNA (species-specific primers)	1100 bp	F- CAT AAC AAT GAG AAT CGC	58	[25]
L. garvieae	16S rRNA (species-specific primers)	1100 bp	R- GCA CCC TCG CGG GTTG	58	[25]
S. pluranimalium	16S rRNA (species-specific primers)	1500 bp	F- AGA GTT TGA TCC TGG CTC AG	52	[26]
S. pluranimalium	16S rRNA (species-specific primers)	1500 bp	R- ACG GCT ACC TTG TTA CGA CTT	52	[26]
A. viridans	16S rRNA gene (species-specific primers)	540 bp	F- GTG CTT GCA CTT CTG ACG TTA GC	55	[27]
A. viridans	16S rRNA gene (species-specific primers)	540 bp	R- TGA GCC GTG GGC TTT CAC AT	55	[27]
S. anginosus	MIL (pbp2b) (Anginosus Group specific)	275 bp	F- TGC TGC AAC GGT AGC TAA TGG	58	[28]
	MIL (pbp2b) (Anginosus Group specific)	275 bp	R- GAA AGG TTT CTG CTG TCC CTG	58
	16srRNA (Species-specific primers)	105 bp	F- GCG TAG GTA ACC TGC CTA TTA GA	58
	16srRNA (Species-specific primers)	105 bp	R- CGC AGG TCC ATC TAC TAGC	58

ISSN: 1746-6148