Table 1 Oligonucleotide primers sequences and size of the PCR-targeted products
| Microorganism | Target Gene | Bp fragment | Primer sequence (5′ - 3′) | Annealing Temp (°C) | References |
|---|---|---|---|---|---|
| E. faecalis / E. gallinarum |
16S rRNA (Genus -specific primers) | 112 bp | F- TAC TGA CAA ACC ATT CAT GAT G |
59 (E. faecalis) 50 (E. gallinarum) | [23] |
| R- AAC TTC GTC ACC AAC GCG AAC | |||||
| E. faecalis |
ddl
E.fecalis
(Species-specific primers) | 941 bp | F- ATC AAG TAC AGT TAG TCT | 55 | [24] |
| R- ACG ATT CAA AGC TAA CTG | |||||
| L. garvieae |
16S rRNA (species-specific primers) | 1100 bp | F- CAT AAC AAT GAG AAT CGC | 58 | [25] |
| R- GCA CCC TCG CGG GTTG | |||||
| S. pluranimalium |
16S rRNA (species-specific primers) | 1500 bp | F- AGA GTT TGA TCC TGG CTC AG | 52 | [26] |
| R- ACG GCT ACC TTG TTA CGA CTT | |||||
| A. viridans |
16S rRNA gene (species-specific primers) | 540 bp | F- GTG CTT GCA CTT CTG ACG TTA GC | 55 | [27] |
| R- TGA GCC GTG GGC TTT CAC AT | |||||
| S. anginosus |
MIL (pbp2b) (Anginosus Group specific) | 275 bp | F- TGC TGC AAC GGT AGC TAA TGG | 58 | [28] |
| R- GAA AGG TTT CTG CTG TCC CTG | |||||
|
16srRNA (Species-specific primers) | 105 bp | F- GCG TAG GTA ACC TGC CTA TTA GA | 58 | ||
| R- CGC AGG TCC ATC TAC TAGC |