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Table 1 Oligonucleotide primers sequences and size of the PCR-targeted products

From: Characterization and susceptibility of streptococci and enterococci isolated from Nile tilapia (Oreochromis niloticus) showing septicaemia in aquaculture and wild sites in Egypt

Microorganism Target Gene Bp fragment Primer sequence (5′ - 3′) Annealing Temp (°C) References
E. faecalis / E. gallinarum 16S rRNA
(Genus -specific primers)
112 bp F- TAC TGA CAA ACC ATT CAT GAT G 59 (E. faecalis)
50 (E. gallinarum)
[23]
R- AAC TTC GTC ACC AAC GCG AAC
E. faecalis ddl E.fecalis
(Species-specific primers)
941 bp F- ATC AAG TAC AGT TAG TCT 55 [24]
R- ACG ATT CAA AGC TAA CTG
L. garvieae 16S rRNA
(species-specific primers)
1100 bp F- CAT AAC AAT GAG AAT CGC 58 [25]
R- GCA CCC TCG CGG GTTG
S. pluranimalium 16S rRNA
(species-specific primers)
1500 bp F- AGA GTT TGA TCC TGG CTC AG 52 [26]
R- ACG GCT ACC TTG TTA CGA CTT
A. viridans 16S rRNA gene
(species-specific primers)
540 bp F- GTG CTT GCA CTT CTG ACG TTA GC 55 [27]
R- TGA GCC GTG GGC TTT CAC AT
S. anginosus MIL (pbp2b)
(Anginosus Group specific)
275 bp F- TGC TGC AAC GGT AGC TAA TGG 58 [28]
R- GAA AGG TTT CTG CTG TCC CTG
16srRNA
(Species-specific primers)
105 bp F- GCG TAG GTA ACC TGC CTA TTA GA 58
R- CGC AGG TCC ATC TAC TAGC