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Table 1 Comparison of standard IF-F and IF-FFPE after Try-30/MW-TEB pretreatment

From: Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogs

 

IgG

IgA

IgM

C3

TEM

Case

Diagnosis

IF-FFPE

IF-F

IF-FFPE

IF-F

IF-FFPE

IF-F

IF-FFPE

IF-F

Dens bodies

1

ICGN

+++

+++a

+

+

+

+

+++

++

+++b/sepi

2

ICGN

+++

+++

+

+

+

−/+

++

+++

+++/sepi

3

ICGN

++

+

−/+

+

−/+

+

++

++

++/sepi

4

ICGN

+

+

+

+

+

+

+/pme and sendo

5

ICGN

−/+

−/+

+

−/+

++

++

−/+

+

+/pme and sendo

6

ICGN

−/+

−/+

−/+

−/+

+

−/+

+

+/pme and sendo

7

ICGN

−/+

+++

+++

+

+

+

+

+/me

8

ICGN

+

+

+++

+++

−/+

+

+

+/me

9

Non-ICGN

−/+

−/+

−/+

+

−/+

−/+

10

Non-ICGN

−/+

−/+

+

−/+

−/+

−/+

11

Non-ICGN

12

Amyloidosis

−/+

−/+

−/+

  1. a: severity of granular signals in glomeruli; b: severity of dens bodies. TEM: transmission electron microscopy, sepi subepithelial areas in the glomerular capillaries, pme paramesangial areas in the glomeruli, sendo subendothelial areas in the glomerular capillaries. me mesangial areas in the glomeruli