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Table 3 Comparative performance of LFD-RPA, PCR and SYBR Green I real time qPCR assays for detection of IBRV suspected clinical specimens

From: Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays

Samples name PCR LFD-RPA Real-time qPCR LFD-RPA/Real-time qPCR LFD-RPA/ PCR/ Real-time qPCR
P N P N P N P N P N
fecal 18 6 20 4 20 4 20 4 18 4
blood 30 6 32 4 32 4 32 4 30 4
nasal swab 20 18 24 14 24 14 24 14 20 14
lymph node 3 0 3 0 3 0 3 0 3 0
spleen 3 0 3 0 3 0 3 0 3 0
lung 2 0 2 0 2 0 2 0 2 0
Total 76 30 84 22 84 22 84 22 76 22
  1. Note: P Positive; N Negative