Table 1 The sequences of primers and probes designed in this study
Primers | Oligonucleotide sequences (5’to3’) | Product sizes (bp) | Location |
|---|---|---|---|
1F: | 5′-GGCAAGCGCCGCGAAGGAAACCAAGTCG-3′ | 236 | 4013–7237 |
1R: | 【Biotin】 5′-GGCTTTACTCCAACAGCGGTGCGACTCCCTTC-3’ | ||
1LF: | 5′-【FAM】GCGAAGGAAACCAAGTCGGGGAAGGCGAGTC[dSpacer]CGAGGGGTTAGGCG【C3-spacer】-3’ | ||
2F | 5′- GGGGACGGCCACGTACACTTGCTCGTCGTAGAGG −3′ | 187 | 19,597–20,823 |
2R: | 【Biotin】5′- TCTGTCCGACGGCGGCAACGGCAACCCAATC −3’ | ||
2-1LF1: | AAAGGCGTTTCGCAGCGAGGCGCCAAAGGG[dSpacer]CTCAGCAGGGCGGAGAC-3′ | ||
2-2LF2: | 5′-【FAM】AGGCGTTTCGCAGCGAGGCGCCAAAGGGG[dSpacer]TCAGCAGGGCGGAGACGT【C3-spacer】-3’ | ||
3F: | 5′- ACACGAGGTCGCCGCCCGTGAAGACGCCGTTGTT −3′ | 142 | 23,526–25,889 |
3R | 【Biotin】5′- CCAATGGACGGAAAGGTGCCCGCCTCCCAGAT −3’ | ||
3LF: | 5′-【FAM】CCGTTGTTGGTGGCCTTGCGGATCTTGCAG[dSpacer]AGTAGAGCCCCGTCTTA【C3-spacer】-3’ | ||
4-1F: | 5′- TTGACAAAGACCGAGTCCGTGTCGCCGTAAACCA −3′ | 274 | 45,238–48,978 |
4-1R: | 【Biotin】5′- TCGATAAGCAGCAGGCCGCCATCAAGGTCGTGT −3′ | ||
4-1LF: | 5′-【FAM】TGTGGCCCAGCGCTCGTGTATGTACCGCCG[dSpacer]GTCTCGAGCAGCATGTC【C3-spacer】-3’ | ||
4-2F | 5′-GCTTTCCGACCAGCGCCGAATCTTGGATGCAGT −3′ | 250 | 47,151–47,397 |
4-2R: | 【Biotin】5′- GGCCAAAACCGCTTTCAGAAGCAGAGCAAGGTGA −3’ | ||
4-2LF: | 5′-【FAM】CCGCAAAGTGCCGAGGGATGTCCTTGTAGT[dSpacer]CAGGTCCACCTTCCGCT【C3-spacer】-3’ | ||
4-3F: | 5′- GCCACCGCCGACAGCTCCAGGTGGGGCAAGTAC −3′ | 295 | 47,151–47,397 |
4-3R: | 【Biotin】5′- CGGGCCAAAACCGCTTTCAGAAGCAGAGCAAGGT −3’ | ||
4-3LF: | 5′-【FAM】CACCTTCCGCTCCCGCAGGGCCTCCTCGGC[dSpacer]ACGGCGTTGAGCTTGTA【C3-spacer】-3′ | ||
4-4F | :5′- CTTTCCGACCAGCGCCGAATCTTGGATGCAGTA −3′ | 154 | 47,151–47,397 |
4-4R: | 【Biotin】5′- CAGCTACAAGCTCAACGCCGTGGCCGAGGAGGC −3’ | ||
4-4LF: | 5′-【FAM】CCGCAAAGTGCCGAGGGATGTCCTTGTAGT[dSpacer]CAGGTCCACCTTCCGCTC【C3-spacer】-3′ | ||
gB-F: | 5′- ACACGGACCGCGTGCCCGTGGGCATGGGCGAGATCA −3′ | 257 | 55,015–58,579 |
gB-R: | 【Biotin】5′- GCAGTTCACAGAGGTGCCCGTGCGGTAGAGCC −3’ | ||
gB-LF: | 5′-【FAM】AGCGGGCGCAAGGTGGTGGCCTTTGACCGC[dSpacer]ACGACGACCCCTGGGAG【C3-spacer】-3′ |