Fig. 2
Sensitivity of the LFD-RPA assay. a Molecular sensitivity of RPA using total DNA extracted from 10-fold serially diluted IBRV-infected cell as template. IBRV templates of lane 1 to 10 in these reactions extracted from ten-fold serially diluted virus range from 5 × 108 to 5 × 10−1 DNA copies per reaction. Samples were tested in triplicate with one reaction displayed in figure for each triplicate. b IBRV RPA reaction products (247 bp) could be detected on a 2% agarose gel. Lane 1 to 9 were represented IBRV RPA reactions extracted from ten-fold serially diluted IBRV templates range from 5 × 106 to 5 × 10−1 DNA copies per reaction. c Repeatability of limits detection. The sensitivity of the assay using IBRV DNA extracted from 5 copies per reaction of IBRV DNA and negative control (DNase-free water) were also evaluated in 3 replicates, respectively. d Sensitivity of conventional PCR. Molecular sensitivity of conventional PCR uses the same template amount as the IBRV LFD-RPA assay. Templates of lane 1 to 10 in these reactions extracted from ten-fold serially diluted virus genome DNA range from 5 × 108 to 5 × 10−1copies per reaction. Samples were tested in triplicate with one reaction displayed in figure for each triplicate