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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays

Fig. 1

Validating the designed primers/probe of IBRV LFD-RPA assay. a Agarose gel electrophoresis of RPA products generated using designed primers/probes. Lane M: molecular weight standard (DNA Marker1000). Lane 1 to 9: designed primer and probe sets. Especially, Lane 5 was primers/probe set 4-2F/4-2R/4-2LF, and the expected size of the product was 247 bp. b Lateral-flow strip end-point analysis of RPA products generated using optimal designed primers/probes set 4F/4R/4LF. Lane 1: positive control (supplied by Twist Ampnfo kit); Lane 2: negative control (DNase-free water); Lane 3: IBRV amplicons performed with RPA primer pair 4F/4R/4LF. Samples were tested in triplicate with one reaction displayed in figure for each triplicate

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BMC Veterinary Research

ISSN: 1746-6148

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