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Table 2 The effect of trypsin pretreatment of PEDV 8aa and time-of-addition assay with trypsin. Concentrated PEDV 8aa was used for this study

From: Trypsin-independent porcine epidemic diarrhea virus US strain with altered virus entry mechanism

 

Pre-treatment of virus with trypsin

Time-of-addition assay (post-inoculation)

Mock

1 μg/ml

5 μg/ml

Mock

1 μg/ml

30 min

1 h

30 min

1 h

0 h

2 h

4 h

12 h

Log10, TCID50/ml

7.23

6.59*

6.26*

6.34*

5.37*

7.10

1.60*

3.50*

6.00*

6.80

  1. For trypsin pretreatment study, virus was incubated with trypsin at 1 or 5 μg/ml for 1 h at 37 °C and added to Vero cells. For time-of-addition assay, trypsin at 1 μg/ml was added to Vero cells infected with PEDV 8aa at the designated time points post infection. Viral titration was done at 24 h post infection for both pre-treatment and time-of-addition assay. Mock indicates treatment with mock-medium. * P < 0.05 compared to the control (Mock)