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Table 2 The effect of trypsin pretreatment of PEDV 8aa and time-of-addition assay with trypsin. Concentrated PEDV 8aa was used for this study

From: Trypsin-independent porcine epidemic diarrhea virus US strain with altered virus entry mechanism

  Pre-treatment of virus with trypsin Time-of-addition assay (post-inoculation)
Mock 1 μg/ml 5 μg/ml Mock 1 μg/ml
30 min 1 h 30 min 1 h 0 h 2 h 4 h 12 h
Log10, TCID50/ml 7.23 6.59* 6.26* 6.34* 5.37* 7.10 1.60* 3.50* 6.00* 6.80
  1. For trypsin pretreatment study, virus was incubated with trypsin at 1 or 5 μg/ml for 1 h at 37 °C and added to Vero cells. For time-of-addition assay, trypsin at 1 μg/ml was added to Vero cells infected with PEDV 8aa at the designated time points post infection. Viral titration was done at 24 h post infection for both pre-treatment and time-of-addition assay. Mock indicates treatment with mock-medium. * P < 0.05 compared to the control (Mock)