Fig. 5

Confocal microscopic study on the effects of trypsin on the entry of PEDV 8aa (P70) into the cells. (a and b) Vero cells were inoculated with mock-medium (Panel 1 to 4) or PEDV 8aa (50 MOI) and incubated at 4 °C for 1 h, then mock-medium (Panels 5 to 8) or trypsin (Panels 9 to 12) were added to the cells. The cells were further incubated at 37 °C for 30 min (a) or 3 h (b) and then fixed for confocal microscopy. Fixed cells were probed with sytox orange (blue) for nucleus, anti-Rab7 antibody (red) for the endosomes or anti-PEDV antibody (green). Merged images of viruses and the endosomes (white) were prepared (Panel 4, 8, 12 in a and b)