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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Trypsin-independent porcine epidemic diarrhea virus US strain with altered virus entry mechanism

Fig. 2

IFA and Western blot analysis of PEDV 8aa (P70) and PEDV KD grown in various culture conditions. a IFA: Vero cells were inoculated with mock-medium (Mock) (Panel 1), PEDV 8aa (P70) (Panels 2–4) or PEDV KD (P70) (Panels 5 and 6). PEDV 8aa-infected cells were further incubated in MEM containing mock (Panel 2), GCDCA (100 μM) (Panel 3) or trypsin (1 μg/ml) (Panel 4). Vero cells inoculated with PEDV KD (P70) (Panels 5 and 6) were further incubated in MEM containing trypsin (1 μg/ml) (Panel 5) or mock (Panel 6). At 24 h post virus infection, cells were fixed for IFA. b Western blot analysis: Vero cells were inoculated with PEDV 8aa (P70) at an MOI of 1, and virus infected cells were incubated with MEM only (M), GCDCA (100 uM) or various concentrations of trypsin (0.01 to 1 μg/ml) for 24 h. After 24 h of incubation, cell lysates were prepared for Western blot analysis for PEDV or β-actin

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