Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach

BMC Veterinary Research

Table 3 Details of primers and qPCR conditions for the putative reference genes assessed in this study

Reference gene	Accession number	Forward primer 5′ to 3′	Reverse primer 5′ to 3′	Product length (bp)	T_a (°C)
RPS5	XM_533568	TCACTGGTGAGAACCCCCT	CCTGATTCACACGGCGTAG	141	62.5
RPS19	XM_533657	CCTTCCTCAAAAAGTCTGGG	GTTCTCATCGTAGGGAGCAAG	95	61
HPRT	AY_283372	AGCTTGCTGGTGAAAAGGAC	TTATAGTCAAGGGCATATCC	114	56
HNRNPH	XM_53857	CTCACTATGATCCACCACG	TAGCCTCCATAACCTCCAC	151	61.2
RPL8	XM_532360	CCATGAATCCTGTGGAGC	GTAGAGGGTTTGCCGATG	64	55
GAPDH	NM_001003142	TGTCCCCACCCCCAATGTATC	CTCCGATGCCTGCTTCACTACCTT	100	58
B2M	XM_535458	TCCTCATCCTCCTCGCT	TTCTCTGCTGGGTGTCG	85	61.2
SRPR	XM_03184	GCTTCAGGATCTGGACTGC	GTTCCCTTGGTAGCACTGG	81	61.2
GUSB	NM_001003191	AGACGTTCCAAGTACCCC	AGGTGTGGTGTAGAGGAGCAC	103	62

ISSN: 1746-6148