Fig. 1

Box and whiskers plot depicting the degree of haemolysis based on absorbance at 414 nm for n = 13 equine plasma samples before and after exercise (cohort A) and from n = 12 equine plasma sampes from horses at rest (cohort C). A value of ≤0.2 absorption units was used as the threshold for acceptable haemolysis and accurate quantification of ci-miRNA detection. The red line indicates the threshold above which accurate quantification of ci-miRNA is not possible