Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia

BMC Veterinary Research

Table 1 Oligonucleotides primers for detection of mycoplasmas and A. laidlawii identified by conventional PCR

Primers	Sequencing 5′-3′	Species	Targets	Amplicon size (bp)	Ref
Mb-F	CTTGGATCAGTGGCTTCATTAGC	M.bovis	VspA gene	400	[1]
Mb-R	GTCATCATGCGGAATTCTTGGGT	M.bovis	VspA gene	400	[1]
Arg-F	AGAGTTTGATCCTGGCTCAGGA	M. arginini	16S rRNA gene	449	[11]
Arg-R	TCAACCAGGTGTTCTTTCCC	M. arginini	16S rRNA gene	449	[11]
Mbv-F	GCTGATAGAGAGGTCTATCG	M. bovirhinis	16S rRNA gene	316	[34]
Mbv-R	ATTACTCGGGCAGTCTCC	M. bovirhinis	16S rRNA gene	316	[34]
Acho-F	AGCCGGACTGAGAGGTCTAC	A. lailawii	16S rRNA gene	505	[11]
Acho-R	TAATCCTGTTTGCTCCCCAC

ISSN: 1746-6148