Fig. 7

MiR-139 also regulates synthesis of β-casein and IGF1R signaling by influencing GHR. BMECs were transfected with (without) GHR siRNA and (or) miR-139 inhibitor. a GHR mRNA levels were examined by qPCR at 24 h post-transfection in cultured BMECs. GHR expression was normalized to β-actin. b Western blotting analysis showed the levels of IGF1R, p-AKT1, AKT1, β-casein, and β-actin at 48 h post-transfection in cultured BMECs. c Relative protein levels were quantified by analyzing scanned blots in panel b using Bandscan5.0 software. β-actin was used as a loading control. For a and c, results are shown as means ± SEM from three independent experiments. Statistical analysis was conducted using one-way ANOVA. Bars with different lowercase letters are significantly different (P < 0.05)