Fig. 3

GHR and IGF1R are target genes of miR-139. BMECs were transfected with negative control of miR-139 mimic (Mimic NC), miR-139 mimic, negative control of miR-139 inhibitor (Inhibitor NC) or miR-139 inhibitor using Lipofectamine 2000. The Control group was only transfected with Lipofectamine 2000. MRNA levels of miR-139 (a, b), GHR (c, d) and IGF1R (e, f) were examined by qPCR at 24 h post-transfection. MiR-139 expression was normalized to U6. GHR and IGF1R expression was normalized to β-actin. Results are shown as means ± SEM from three independent experiments. Statistical analysis was conducted using one-way ANOVA.“*”, statistical significance was considered at P<0.05