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Table 1 Means and standard deviations of ruminal variables in the control group and in the groups supplemented with monensin and yeast culture at various time points

From: Comparative assessment of probiotics and monensin in the prophylaxis of acute ruminal lactic acidosis in sheep

  

Time points

 

p

 

Variable

Groups

T0 h

T6 h

T12 h

T18 h

T24 h

T36 h

T48 h

Trat

Time

Trat*Time

pH

Control

6.60 ± 0.12a

4.81 ± 0.29c

4.45 ± 0.05Bcd

4.50 ± 0.30cd

4.35 ± 0.30d

4.76 ± 0.57Bc

5.70 ± 0.75Bc

0.01

0.01

0.14

Monensin

6.79 ± 0.23a

4.80 ± 0.13c

5.21 ± 0.53Ac

4.96 ± 0.55c

4.79 ± 0.37c

5.47 ± 0.39ABbc

6.41 ± 0.61ABab

   

Yeast

6.72 ± 0.17a

4.87 ± 0.18b

4.80 ± 0.12ABb

4.50 ± 0.35b

4.85 ± 0.77b

6.36 ± 0.59Aa

7.08 ± 0.35Aa

   

Temperature (°C)

Control

38.8 ± 0.6c

40.4 ± 0.5b

41.1 ± 0.2Aa

38.4 ± 0.7cd

38.1 ± 0.6cd

37.8 ± 1.5Bde

37.7 ± 1.5Be

0.03

0.01

0.13

Monensin

38.5 ± 0.6b

40.0 ± 0.5a

40.4 ± 0.2Ba

39.7 ± 0.7ab

39.5 ± 0.6ab

40.2 ± 1.5Aa

40.0 ± 1.5Aa

   

Yeast

39.0 ± 0.2b

40.7 ± 0.6a

40.6 ± 0.4ABa

39.7 ± 1.1ab

39.5 ± 1.6ab

39.9 ± 0.6Aab

40.0 ± 0.4Aab

   

Osmolarity* (mOsm/L)

Control

274 ± 19b

453 ± 75Aa

357 ± 92ab

309 ± 29A

328 ± 67Ab

306 ± 14Ab

309 ± 21Aab

0.02

0.01

0.09

Monensin

253 ± 12

377 ± 89B

284 ± 75

307 ± 81A

339 ± 42A

256 ± 42AB

218 ± 34B

   

Yeast

206 ± 13b

293 ± 42Bab

299 ± 42a

278 ± 77Bab

234 ± 26Bab

205 ± 53Bab

202 ± 22Bab

   

Lactate L (mmol/L)

Control

0.47 ± 0.1d

33.6 ± 11bcd

68.3 ± 27ab

69.4 ± 30ab

81.2 ± 33Aa

47.8 ± 20Aabc

15.2 ± 42Acd

0.01

0.01

0.14

Monensin

0.34 ± 0.1c

30.3 ± 12ab

39.9 ± 17ab

42.9 ± 18a

41.6 ± 12ABa

26.2 ± 18Aab

16.2 ± 80Ac

   

Yeast

0.26 ± 0.3c

22.3 ± 52bc

38.9 ± 11ab

51.0 ± 19a

33.0 ± 20Bab

1.39 ± 0.9Bc

0.45 ± 0.1Bc

   
  1. Upper-case letters in columns denote statistically significant differences (p < 0.05) between groups throughout least square means test. Lower-case letters in rows denote statistically significant differences (p < 0.05) between time points by analysis of variance for measurements repeated over time. * Osmolarity presented difference in the T0 h, thus those values were included as covariates in the statistical analyses of this variable