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Table 1 Primer sets used to amplify DNA fragments specific for Babesia bovis

From: In silico and phylogenetic analyses of partial BbRAP-1, BbCP2, BbSBP-4 and BbβTUB gene sequences of Babesia bovis isolates from cattle in South Africa

Gene Assay Primer name Oligonucleotide primers (5′ → 3′) Annealing Product sizea Reference
BbRAP-1 PCR BoF F-CACGAGGAAGGAACTACCGATGTTGA 55 °C 360 bp [12]
BoR R-CCAAGGAGCTTCAACGTACGAGGTCA [12]
nPCR BoFN F-TCAACAAGGTACTCTATATGGCTACC 57 °C 298 bp [12]
BoRN R-CTACCGAGCAGAACCTTCTTCACCAT [12]
BbCP2 PCR CpBovF F-TGCATCGGACCTATCCAACC 57 °C 960 bp This study
CpBovR R-TCAGCAGCCAAATAAGGCCA This study
nPCR CpBov3 F-ATCGGAAGAAGTCGCCGTTG 65 °C 829 bp This study
CpBov4 R-AAGCGTAGTCGCTGTAACCA This study
BbSBP-4 PCR BbSBP1 F-AGTTGTTGGAGGAGGCTAAT 57 °C 887–905 bp This study
BbSBP2 R-CTTCTCGGCGTCCTTTTC This study
nPCR BbSBP3 F-CCGCATTCTTAAGACTTCTGA 60 °C 726–744 bp This study
BbSBP4 R-GTTACCATTTCATCGTTGTCA This study
BbβTUB PCR BTbovA F-AGAGCGGTACTTACCACGGA 61 °C 1203 bp This study
BTbovB R-CGTCGTCGATGGTTGCTTCT This study
nPCR BTbovC F-GTTCCACGCGCTGTACTCAT 65 °C 954 bp This study
BTbovD R-CATGTCCTGGATGGCGGTAG This study
  1. aTheoretical product sizes based on nucleotide gene sequences of several B. bovis strains used as templates for primer design