Gene | Assay | Primer name | Oligonucleotide primers (5′ → 3′) | Annealing | Product sizea | Reference |
---|---|---|---|---|---|---|
BbRAP-1 | PCR | BoF | F-CACGAGGAAGGAACTACCGATGTTGA | 55 °C | 360 bp | [12] |
BoR | R-CCAAGGAGCTTCAACGTACGAGGTCA | [12] | ||||
nPCR | BoFN | F-TCAACAAGGTACTCTATATGGCTACC | 57 °C | 298 bp | [12] | |
BoRN | R-CTACCGAGCAGAACCTTCTTCACCAT | [12] | ||||
BbCP2 | PCR | CpBovF | F-TGCATCGGACCTATCCAACC | 57 °C | 960 bp | This study |
CpBovR | R-TCAGCAGCCAAATAAGGCCA | This study | ||||
nPCR | CpBov3 | F-ATCGGAAGAAGTCGCCGTTG | 65 °C | 829 bp | This study | |
CpBov4 | R-AAGCGTAGTCGCTGTAACCA | This study | ||||
BbSBP-4 | PCR | BbSBP1 | F-AGTTGTTGGAGGAGGCTAAT | 57 °C | 887–905 bp | This study |
BbSBP2 | R-CTTCTCGGCGTCCTTTTC | This study | ||||
nPCR | BbSBP3 | F-CCGCATTCTTAAGACTTCTGA | 60 °C | 726–744 bp | This study | |
BbSBP4 | R-GTTACCATTTCATCGTTGTCA | This study | ||||
BbβTUB | PCR | BTbovA | F-AGAGCGGTACTTACCACGGA | 61 °C | 1203 bp | This study |
BTbovB | R-CGTCGTCGATGGTTGCTTCT | This study | ||||
nPCR | BTbovC | F-GTTCCACGCGCTGTACTCAT | 65 °C | 954 bp | This study | |
BTbovD | R-CATGTCCTGGATGGCGGTAG | This study |