Table 1 SiRNA for silencing as well as primers for plasmid construction and real-time PCR used in this study
Purpose | Name | Sequence(5’ to3’) | Accession no. | References |
|---|---|---|---|---|
Cloning of chMDA5 | chMDA5-F1 | AAAGATATCTATGTCGGAGGAGTGCCGA (EcoRV) | GU570144.1 | Â |
chMDA5-R1 | AATGGATCCCTTCTTTTGTCATC | Â | Â | |
Cloning of chMDA5 | chMDA5-F2 | ACAAAAGAAGGGATCCATTTAGAG (overlap sequence) | Â | Â |
chMDA5-R2 | CTAGTCTAGATTAATCTTCATCACTTGAAGGACAA (XbaI) | Â | Â | |
Cloning of chTLR3 | chTLR3-F | ATAAGAATGCGGCCGCTAAACTAATGGGATGCTCTATTCCTTGCT (NotI) | NM_001011691 | Â |
chTLR3-R | AAAGATATCAATCAGCGCACTTTACTATTAGATTTAAG (EcoRV) | Â | Â | |
Cloning of dgRIG- I | dgRIG-I-F | GGAATTCC ATGACGGCGGAGGAAAAG (EcoRI) | JF804977.1 | Â |
dgRIG-I-R | GAGGATCCTCAAATGGTGGGTACAAGTTGGAC (BamHI) | Â | Â | |
Silencing | SiMDA5 | GAACGUGAAGAUGUAAAUATT | Â | [22] |
Silencing | SiTLR3 | GCAGAUUGUAGUCACCUAATT | Â | [22] |
Silencing | SiMAVS | UACAGGAGGCUUCAAGGAGGUGUCA | Â | [22] |
Silencing | siRNA control | AUUACGGGCCAGUAAUCUAT | Â | Â |
Real-time PCR | chIFN-β F | CAGCTCTCACCACCACCTTCTC |  | [24] |
chIFN-β R | GGAGGTGGAGCCGTATTCTG |  |  | |
Real-time PCR | chβ-actin F | CAACACAGTGCTGTCTGGTGGTA |  | [27] |
chβ-actin R | ATCGTACTCCTGCTTGCTGATCC |  |  | |
Real-time PCR | chIFN-λ F | TGAGCTGGACCTCACCATCA | NM_001128496.1 |  |
Real-time PCR | chIFN-λ R | GGGCTGTTGGCACGTCTCT |  |  |
Real-time PCR | chMda5 F | TGGAGCTGGGCATCTTTCAG | Â | [23] |
chMda5 R | GTTCCCACGACTCTCAATAACAGT | Â | Â | |
Real-time PCR | chMx F | TTGTCTGGTGTTGCTCTTCCT | Â | [25] |
chMx R | GCTGTATTTCTGTGTTGCGGTA | Â | Â | |
Real-time PCR | IBV-GL533 | GCCATGTTGTCACTGTCTATTG | Â | [26] |
IBV-GU391 | GCTTTTGAGCCTAGCGTT | Â | Â | |
IBV-Probe | FAM-CACCACCAGAACCTGTCACCTC-BHQ | Â | Â |