Fig. 3

IBV induces high expression of chMDA5, chIFN-β, chIFN-λ and chMx in CEK cells. CEK cells were infected with IBV at an MOI of 1. At the indicated times post-infection, cells were harvested for RNA isolation, and virus-induced expression of chMDA5 (a), chIFN-β (b), chIFN-λ (c) and chMx (d) was determined by RT-qPCR. All gene expression was calculated as the fold change relative to mock cells uninfected in parallel and normalized against a housekeeping gene (β-actin). Data are presented as the mean ± SD (n = 3, 3 wells on same plate) (* P ≤ 0.05; ** P ≤ 0.01)