Local anaesthetics or their combination with morphine and/or magnesium sulphate are toxic for equine chondrocytes and synoviocytes in vitro

Table 2 Primer sequences of the reference gene (GAPDH) and pro-inflammatory cytokines IL1β, IL6 and TNFα used in the study

Gene	GeneBank Accession	Direction	Primer Sequence (5′-3′)
GAPDH	NM_001163856	Forward	GCATCGTGGAGGGACTCA
GAPDH	NM_001163856	Reverse	GCCACATCTTCCCAGAGG
IL-1β	NM_001317261	Forward	GCCTAAGAATACTACATCCAGAGA
IL-1β	NM_001317261	Reverse	GGCATTGATTAGACAACAGTGAA
IL-6	NM_001082496	Forward	CTGCTCCTCGTGATGGCTAC
IL-6	NM_001082496	Reverse	CCGAGGATGTACTTAATGTGCTG
TNF-α	NM_001081819	Forward	CCTTCCACTCAATCAACCCTCT
TNF-α	NM_001081819	Reverse	CACGCCCACTCAGCCACT

Primer sequences of the reference gene (GAPDH) and proinflammatory cytokines IL1β, IL6 and TNFα used in the study

ISSN: 1746-6148