Fig. 3

Results of flow cytometric analysis. a. Bar chart showing mean values of immunofluorescence of MHC class II (box filled with diagonal pattern), CD44 (solid black box), CD86 (solid gray box), and CD172α (white box) expression in peripheral blood mononuclear cell (PBMCs), blood monocyte (B-MOS), and blood dendritic cell (B-DC), as well as lung mononuclear cell (L-MC), lung monocyte (L-MOS), and lung dendritic cell (L-DC). The error bars indicate standard deviation. b. Representative results showing dot plots of blood cells (PBMC, B-MOS, and B-DC) from one horse and lung cells (L-MC, L-MOS, and L-DC) from another horse. The X-axis for each dot plot is a logarithmic scale of immunofluorescence parameter (FL1) for MHC class II, CD44, CD86, CD172a, and isotype controls including IgG1 and IgG2b; the y-axis for each dot plot is forward scatter (FSC), indicating the size of the cells