Table 2 Sequences of primers and probes for CPV-2 PCR, real-time PCR and real-time RPA assay
Name | Sequence 5′-3’Amplication size (bp) | |
|---|---|---|
VP2-FP | ATGAGTGATGGAGCAGTTCAACCAGAC | 1775 |
VP2-RP | TTAATATAATTTTCTAGGTGCTAGTTGA | |
CPV-FP | AAACAGGAATTAACTATACTAATATATTTA | 93 |
CPV-RP | AAATTTGACCATTTGGATAAACT | |
CPV-P | FAM-TGGTCCTTTAACTGCATTAAATAATG TACC-BHQ1 | |
CPV-RPA-FP | CACTTACTAAGAACAGGTGATGAATTTGCT ACAG | 214 |
CPV-RPA-RP | AGTTTGTATTTCCCATTTGAGTTACACCACGTCT | |
CPV-RPA-P | CCTCAAGCTGAAGGAGGTACTAACTTTGGT/ BHQ1-dT//THF //FAM-dT/ATAGGAGTTCAAC AAG-C3 spacer |