TY - JOUR AU - Ledesma, Darién AU - Berriatua, Eduardo AU - Thomas, M. Carmen AU - Bernal, Luis Jesús AU - Ortuño, María AU - Benitez, Celia AU - Egui, Adriana AU - Papasouliotis, Kostas AU - Tennant, Bryn AU - Chambers, Julia AU - Infante, Juan José AU - López, Manuel Carlos PY - 2017 DA - 2017/10/23 TI - Performance of Leishmania PFR1 recombinant antigen in serological diagnosis of asymptomatic canine leishmaniosis by ELISA JO - BMC Veterinary Research SP - 304 VL - 13 IS - 1 AB - Leishmania infantum is a protozoan parasite transmitted by phlebotomine sand flies that causes life-threatening disease in humans and dogs. The dog is the primary reservoir of the parasite and early diagnosis of canine leishmaniosis is crucial at the clinical and epidemiological level. The currently available serological tests for CanL diagnostic show limitations therefore the aim of the present study was to investigate the diagnostic performance of an indirect antibody ELISA based on the Leishmania infantum recombinant antigen PFR1 in asymptomatically infected dogs. One hundred fifty-six dogs including Leishmania-free experimental Beagles and pet dogs from England, Scotland and Leishmania-endemic Murcia in Spain, were tested with the assay. The later were also tested with two commercial L. infantum crude antigen ELISAs (INgezim and Civtest, respectively) and a real-time kinetoplast PCR test. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-017-1224-z DO - 10.1186/s12917-017-1224-z ID - Ledesma2017 ER -