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Table 3 Microorganisms, sequences and references for the primers used

From: Effects of dietary supplementation of leaves and whole plant of Andrographis paniculata on rumen fermentation, fatty acid composition and microbiota in goats

Microorganism

Sequence 5′ – 3′

Product size (bp)

Annealing temperature (°C)

Reference

Total bacteria F

CGGCAACGAGCGCAACCC

145

55

[18]

Total bacteria R

CCATTGTAGCACGTGTGTAGCC

Total protozoa F

CTTGCCCTCYAATCGTWCT

223

55

[19]

Total protozoa R

GCTTTCGWTGGTAGTGTATT

Methanogens (mcrA)-F

TTCGGTGGATCDCARAGRGC

140

55

[20]

Methanogens (mcrA)-R

GBARGTCGWAWCCGTAGAATCC

Fibrobacter succinogenes

GTTCGGAATTACTGGGCGTAAA

122

55

[21]

Fibrobacter succinogenes

CGCCTGCCCCTGAACTATC

Ruminococcus albus

CCCTAA AAGCAGTCTTAGTTCG

170

55

[18]

Ruminococcus albus

CCTCCTTGCGGTTAGAACA

Ruminococcus flavefaciens F (Rf154f)

TCTGGAAACGGATGGTA

259

55

[18]

Ruminococcus flavefaciens (Rf425r)

CCTTTAAGACAGGAGTTTACAA

  1. F Forward, R Reverse