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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection

Fig. 3

PRRSV receptors and infectivity analysis of Marc-145 cells treated with curcumin before or after infection. Marc-145 cells were incubated with various curcumin concentrations at 37 °C for 1 h. CD163 (a), MYH9 (b) and vimentin (c) protein levels were measured by western blot. Marc-145 cells were incubated with curcumin before addition of GD-HD (MOI: 0.1, d, e) or after infection (f, g) with indicated curcumin concentrations. After incubation at 37 °C for 1 h, cells were maintained in medium without curcumin for 36 h. Next, PRRSV N protein expression levels (d and f) and viral production (e and g) were determined by western blot and virus titration assay, respectively. Each value represents the mean ± SD from three independent experiments (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ND means non-detectable)

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