Table 2 List of oligonucleotide primers used in this study
Target gene | Primers | Sequence (5′ → 3′) | Amplicom size (bp) | Multiplex PCR assay | Reference |
|---|---|---|---|---|---|
16S rRNA a | Forward | ACCGCACTTTAGTGTGTGTG | 816 | Yes | (25) |
| Â | Reverse | TCTCTACGCCGATCTTGTAT | Â | Â | Â |
rpoB a | Forward | CGTATGAACATCGGCCAGGT | 446 | Yes | (26) |
| Â | Reverse | TCCATTTCGCCGAAGCGCTG | Â | Â | Â |
pld a | Forward | ATAAGCGTAAGCAGGGAGCA | 203 | Yes | (14) |
| Â | Reverse | ATCAGCGGTGATTGTCTTCCAGG | Â | Â | Â |
narG a | Forward | ACCCGTACTTGCACTCTTTC | 612 | Yes | Present Study |
| Â | Reverse | AGTCAGTACTTCCGCAGGTC | Â | Â | Â |
narT | Forward | GCTGAAGCAAGTTCGTGTCT | 202 | No | Present Study |
| Â | Reverse | GTAACGGTCAGAGAACCATCC | Â | Â | Â |
narK | Forward | GCTGAAGCAAGTTCGTGTCT | 202 | No | Present Study |
| Â | Reverse | GTAACGGTCAGAGAACCATCC | Â | Â | Â |
narG2 | Forward | CAACGTGGTACCTGGTATCTG | 200 | No | Present Study |
| Â | Reverse | CATAGGGAGAGCGAGAACAA | Â | Â | Â |
narH | Forward | GATTCTACTGACCGCCATCTC | 196 | No | Present Study |
| Â | Reverse | ATCAGTACCTGTCATGCCTACC | Â | Â | Â |
narJ | Forward | CGTGATGGTATAGAGGTGCTG | 198 | No | Present Study |
| Â | Reverse | GTTGGAAGCAGTAGGGAAGGGAG | Â | Â | Â |
narI | Forward | CTGTATCCACACAGGTGTTCG | 215 | No | Present Study |
| Â | Reverse | GTATCCTACAGGCGCTGAGA | Â | Â | Â |