Fig. 4

Quantification of plasma HfIL-1β in non-infected and MG-infected house finches using the developed ELISA system. Plasma samples were collected from two individual birds from non-infected and MG-infected groups. Then, plasma samples were serially diluted with PBS as follows: 1:10, 1:20, 1:40, 1:80, and 1:160 (a). The diluted samples were coated with the dehydration method (60°C for 2 h), followed by sequential incubation with anti-ChIL-1β antibody (1:1000) as the primary antibody and the secondary antibody goat anti-rabbit antibody (1:2000). The HRP signal was developed with TMB solution for 30 min. The concentration of HfIL-1β plasma level was calculated using Excel (Microsoft Corp) (b). The values represent the mean of triplicate wells with standard deviation bars. The dashed line indicates the threshold line, representing the value of negative control and limitation of the developed ELISA system (OD450 = 0.042)