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Table 1 Fraction of patent cartilage canal segments characterized by different features

From: Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis

Pig age

82-day old

96-day old

127-day old

All three

(1) No change/total number of segments (%)

87/210 (41)

46/132 (35)

34/70 (49)

167/412 (41)

(2) Surrounded by intense SHG/total number of segments (%)

123/210 (59)

86/132 (65)

36/70 (51)

245/412 (59)

(3) Presence of collagen fibres/total number of segments (%)

59/210 (28)

36/132 (27)

17/70 (24)

112/412 (27)

(4) Indication of calcification/total number of segments (%)

22/210 (10)

8/132 (6)

0/70

30/412 (7)

  1. Fraction of segments that (1) showed no change in SHG signal of the adjacent cartilage matrix as a function of distance from the canal, (2) was surrounded by cells associated with intense SHG signal, (3) had presence of collagen fibres at the canal margin, (4) was surrounded by high TPEF signal in the surrounding cartilage matrix as an indication of calcification