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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis

Fig. 5

Patent cartilage canals crossing the osteochondral junction. Cyan: Two-photon-excited fluorescence (TPEF), Glowing red: Second harmonic generation (SHG). a A cartilage canal with a segment having intact blood vessels (asterisk) is narrowed and reduced to a streak at the ossification front (arrow). The streak was present in the entire 100 μm-section, indicating it was not an artefact. The canal is partly located outside the image plane. The narrowed segment is surrounded by intense SHG signal, clusters of hypertrophic cells, and more distant, a matrix of high TPEF signal indicating calcification. b A cartilage canal surrounded by layers of intense SHG signal, interpreted as collagen fibres or osteoid (arrow). The canal is not narrowed, and its lumen is connected with the bone marrow. 25×–focusing objective was used in both images. Bars equal 250 μm

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