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Table 1 Results of different parameters in the different experimental groups

From: Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains

Parameter

Experimental Groups

   
 

NCG (# = 5)

PCG (# = 20)

VG (# = 20)

P-value

RDS

 D1–53

0 ± 0 a

0.68 ± 0.41b

0.32 ± 0.86 c

<0.001

 D1–23

0 ± 0 a

0 ± 0 a

0.017 ± 0.060 a

0.983

 D24–53

0 ± 0 a

1.20 ± 0.83 b

0.55 ± 0.42 c

<0.001

Weight ± SD

 D0

7.04 ± 1.22a

6.97 ± 0.84 a

7.04 ± 1.02 a

0.966

 D24

15.25 ± 3.96 a

16.39 ± 2.16 a

15.61 ± 2.96 a

0.581

 D53

36.51 ± 8.63 a

34.63 ± 6.63 a

34.27 ± 5.35 a

0.808

ADG (g/pig/d)

 D0–24

357 ± 123 a

393 ± 62.8 a

357 ± 92.0 a

0.372

 D24–53

733 ± 194 a

629 ± 199 a

643 ± 102 a

0.504

 D0–53

563 ± 144 a

522 ± 121 a

514 ± 87.0 a

0.714

MLL, histopathology and air analysis D53

 MLL

0 ± 0 a

7.56 ± 4.73 b

0.68 ± 0.73 a

<0.001

 Histopathology

1.31 ± 0.18 a

3.32 ± 0.85 b

1.92 ± 0.47 c

<0.001

 Percentage of air (%)

47.72 ± 7.29 a

34.49 ± 8.22 b

45.23 ± 5.99 a

<0.001

log copies of M. hyopneumoniae DNA/ml BALF (qPCR) ± SD

 D39

0.36 ± 0.64 a

1.81 ± 1.32 a

1.23 ± 1.61 a

0.062

 D53

0.40 ± 0.43 a

3.99 ± 1.20 b

1.78 ± 1.36 a

<0.001

Percentage of M. hyopneumoniae qPCR positive samples in BALF (#positive samples/total number of samples)

 D39

(0/4) 0a

(14/20) 70b

(7/20) 35c

<0.01

 D53

(0/4) 0a

(19/20) 95b

(12/20) 60c

<0.001

M. hyopneumoniae specific AB expressed in OD-values ± SD in serum

 D1

1.51 ± 0.037 a

1.48 ± 0.058 a

1.48 ± 0.16 a

0.578

 D24

0.91 ± 0.044 a

0.95 ± 0.069 a

0.50 ± 0.17 b

<0.001

 D53

0.90 ± 0.10 a

0.23 ± 0.11 a

0.087 ± 0.041 b

<0.001

Percentage of ELISA M. hyopneumoniae positive samples in serum

 D1

(0/5) 0a

(0/20) 0a

(0/20) 0a

1.000

 D24

(0/4) 0a

(0/20) 0a

(15/20) 75b

<0.001

 D53

(0/4) 0a

(20/20) 100b

(20/20)100b

<0.001

  1. The respiratory disease score (RDS), body weight, average daily gain (ADG), M. hyopneumoniae specific antibodies, macroscopical lung lesions (MLL), histopathology and air analysis, log copies of M. hyopneumoniae DNA (qPCR), percentage of of M. hyopneumoniae qPCR positive samples, M. hyopneumoniae specific AB expressed in OD-values and Percentage of ELISA M. hyopneumoniae positive samples
  2. a,b,cDifferent superscripts in one row are statistically different (P < 0.05)
  3. NCG negative control group, PCG positive control group, VG vaccination group, SD standard deviation, D Day of the study, ADG average daily gain, kg kilogram, RDS respiratory disease score, M. hyopneumoniae Mycoplasma hyopneumoniae, AB antibodies, OD optical densities, # number, MLL macroscopic lung lesions, qPCR quantitative polymerase chain reaction, DNA DeoxyriboNucleic Acid, BALF bronchoalveolar lavage fluid