Fig. 2
Performance of the CDV RT-RPA assay. a Fluorescence development over time using a dilution range of 9.4 × 105 to 9.4 × 10−1 copies of the CDV standard RNA. Numbers for amplification curves were designated as, 1: 9.4 × 105 copies; 2: 9.4 × 104 copies; 3: 9.4 × 103 copies; 4: 9.4 × 102 copies; 5: 9.4 × 101 copies; 6: 9.4 × 100 copies; 7: 9.4 × 10−1 copies. b Semi-logarithmic regression of the data collected from eight CDV RT-RPA tests on the RNA standards using Prism Software 5.0. The run time of the RT-RPA was between 3 min–12 min for CDV RNA from 9.4 × 105 to 9.4 × 100 copies. c Probit regression analysis using SPSS software on data of eight runs. The detection limit at 95% probability (31.8 molecules) is depicted by a rhomboid