Fig. 7
From: Development of duplex PCR for differential detection of goatpox and sheeppox viruses
Single PCR and duplex PCR for detection different GTPV and SPPV nucleic acid using E10R primers and RPO132 primers or mix primers. Lane 1–3: single PCR detect 100 ng SPPV gDNA, 100 ng GTPV gDNA and no template using RPO132 primers; Lane 4–6: single PCR detect 100 ng SPPV gDNA, 100 ng GTPV gDNA and no template using E10R primers; Lane 7–9: duplex PCR detect 100 ng SPPV gDNA, 100 ng GTPV gDNA and no template using mixture of RPO132 primers and E10R primers. Lane M: 2000 bp DNA Ladder Marker (TaKaRa, Dalian)