Fig. 2From: Development of duplex PCR for differential detection of goatpox and sheeppox virusesOptimization of annealing temperature (Tm) for duplex PCR reaction in the detection of GTPV or SPPV using mix primers. Agarose gel electrophoresis showing the effect of Tm on duplex PCR. a Duplex PCR amplicated products using 100 ng SPPV gDNA as template. b Duplex PCR amplicated products using 100 ng GTPV gDNA as template. Lane 1–6 is 46 °C, 48 °C, 50 °C, 52 °C, 54 °C and 56 °C, respectively; Lane M: 2000 bp DNA Ladder Marker (TaKaRa, Dalian) and Lane C: No template controlBack to article page