Fig. 2
From: The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China
PCR amplification and phylogenetic analyses of ORFV011 (B2L) and ORFV059 (F1 L) of the HN3/12 strain. a Amplification of major envelope genes (ORFV 011 and ORFV 059) by PCR. ORFV011 (lane 1, 2, 4) and ORFV059 (lane 5, 6, 8) were amplified from genomic DNA isolated from HN3/12. Lane 3 and 7: DNA extracted from NA1/11 strain as a positive control. Lane M: 2 kb DNA ladder. Phylogenetic analysis of different ORFV strains around the world, based on nucleotide sequences of ORFV011 (b) and ORFV059 (c). The phylogenetic trees were constructed by the neighbor-joining algorithm using MEGA version 5.2 software. One thousand bootstrap replicates were subjected to nucleotide sequence distance (cut-off value of 50% from 1000 bootstrap replicates). All bootstrap values are displayed above branches. The scale bar indicates the branch length. Red frame: HN3/12 in this study