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Table 5 Results of the TG-ROC analysis: Cut-off CT-values and Se of qPCR A and B when Sp was adjusted to 100.0%

From: Evaluation of different diagnostic methods for the detection of Mycobacterium avium subsp. paratuberculosis in boot swabs and liquid manure samples

Matrix

qPCR

Cut-off CT

Se [%]

(95% CI)

Sp [%]a

(95% CI)

Boot swab

A

39.30

73.68

(60.34–84.46)

100.00

(80.49–100.00)

B

36.12

50.91

(37.07–64.65)

100.00

(81.47–100.00)

Liquid manure

A

35.10

50.98

(36.60–65.25)

100.00

(80.49–100.00)

B

35.99

50.94

(36.84–64.94)

100.00

(80.94–100.00)

  1. aSp was fixed at 100.0%, the corresponding Se and cut-offs were calculated