Skip to main content

Table 4 Test characteristics of qPCR A and B for analysis of boot swabs and liquid manure samples using the cut-off CT-values as recommended by the manufacturers’ for faecal samples

From: Evaluation of different diagnostic methods for the detection of Mycobacterium avium subsp. paratuberculosis in boot swabs and liquid manure samples

Matrix

qPCR

Cut-off CT a

Test + /Status +b

Se [%]

(95% CI)

Test - /Status -c

Sp [%]

(95% CI)

Boot swab

A

37.00

35/57

61.40

(47.57–74.00)

17/17

100.00

(80.49–100.00)

B

38.00

36/55

65.45

(51.42–77.76)

16/17

94.44

(72.71–99.86)

Liquid manure

A

37.00

32/51

62.75

(48.08–75.87)

16/17

94.12

(71.31–99.85)

B

38.00

36/53

67.92

(53.68–80.08)

15/17

88.24

(63.56–98.54)

  1. aCT-values ≤37.00 (qPCR A) and ≤38.00 (qPCR B) were considered positive and CT-values >37.00 (qPCR A) and >38.00 (qPCR B) were considered negative.
  2. bNumber of test positive samples per total number of assessable samples from MAP-positive herds (n/n).
  3. cNumber of test negative samples per total number of assessable samples from MAP-negative herds (n/n)
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us