Skip to main content

Table 1 The Oligonucleotide primer sequences and PCR conditions used in the present study

From: Fatal infection in three Grey Slender Lorises (Loris lydekkerianus nordicus) caused by clonally related Trueperella pyogenes

Oligonucleotide primers

Sequence

Programa

1. 16S rDNA UNI-L

5′-AGA GTT TGA TCA TGG CTC AG-3′

1

2. 16S rDNA UNI-R (Amplification primer)

5′-GTG TGA CGG GCG GTG TGT AC-3′

3. 16S rDNA-533F

5′-GTG CCA GCM GCC GCG GTA A-3′

4. 16S rDNA-907R (Sequencing primer)

5′-CCG TCA ATT CMT TTG AGT TT-3′

5. Gap-F

5′-TCG AAG TTG TTG CAG TTA ACG A-3′

2

6. Gap-R

5′-CCA TTC GTT GTC GTA CCA AG-3′

7. ERIC1RF

5′-ATG TAA GCT CCT GGG GAT TCA C-3′

3

8. ERIC2

5′-AAG TAA GTG ACT GGG GTG AGC-3′

9. BOXA1R

5′-CTA CGG CAA GGC GAC GCT GAC G-3′

3

10. (GTG)5

5′-GTG GTG GTG GTG GTG-3′

4

12. RAPD primer B

5′- ATC TGG CAG C − 3′

5

13. fusA-F

5′-GCT TCA TCA ACA AGA TGG AC-3′

6

14. fusA-R

5′-CTC GAT TG CGA CGT GG AT-3′

15. tuf-F

5′-GGA CGG TGA TTG GAG AAG AAT GG-3′

7

16. tuf-R

5′-CCA GGT TGA TTA CGC TCC AGA AGA-3′

17. metG-F

5′-GCC GGT TTT GGT GTT CC-3′

8

18. metG-R

5′-GGC CAA ATC TGG GAA TGG-3′

19. gyrA-F

5′-CCA CCA GAT CGA GGT CAT C-3′

9

20. gyrA-R

5′-TCG TCG GCA GTG AAA CGC A-3′

  1. aPCR Program 1: ×1 (95 °C, 600 s), ×30 (95 °C, 30 s, 58 °C, 60 s, 72 °C, 60 s), ×1 (72 °C, 420 s). 2: ×1 (94 °C, 180 s), ×30 (94 °C, 30 s, 50 °C, 40 s, 72 °C, 60 s), ×1 (72 °C, 300 s). 3: ×1 (95 °C, 180 s), ×30 (94 °C, 30 s, 53 °C, 60 s, 70 °C, 480 s), ×1 (72 °C, 960 s). 4: ×1 (95 °C, 180 s), ×30 (94 °C, 30 s, 53 °C, 60 s, 70 °C, 180 s), ×1 (72 °C, 960 s). 5: ×1 (95 °C, 180 s), ×45 (94 °C, 15 s, 34 °C, 60 s, 70 °C, 120 s), ×1 (72 °C, 600 s). 6: ×1 (94 °C, 180 s), ×30 (94 °C, 45 s, 57 °C, 30 s, 72 °C, 90 s), ×1 (72 °C, 420 s). 7: ×1 (94 °C, 180 s), ×30 (94 °C, 45 s, 57 °C, 40 s, 72 °C, 60 s), ×1 (72 °C, 420 s). 8: ×1 (94 °C, 180 s), ×30 (94 °C, 45 s, 52 °C, 30 s, 72 °C, 90 s), ×1 (72 °C, 600 s). 9: ×1 (94 °C, 180 s), ×30 (94 °C, 45 s, 52 °C, 30 s, 72 °C, 90 s), ×1 (72 °C, 600 s)