Table 2 Results of bacteriological examination (BE) and of the RespoCheck bacterial multiplex real-time PCR of BALF samples of calves originating from herds with BRD associated health problems
Agent | No (%) of BALF samples | Diagnostic Sensitivity | Diagnostic Specificity | Cohen’s Kappa Coefficient | ||||
|---|---|---|---|---|---|---|---|---|
BE + PCR + | BE + PCR- | BE PCR + | BE - PCR - | Total | ||||
P. multocida | 53 (33) | 10 (6) | 29 (18) | 68 (43) | 160 | 0.84 (0.73–0.92)a | 0.70 (0.60–0.79) | 0.52 (0.39–0.64) |
M. haemolytica | 46 (28) | 4 (3) | 30 (19) | 80 (50) | 160 | 0.92 (0.81–0.98) | 0.73 (0.63–0.81) | 0.57 (0.45–0.69) |
H. somni | 5 (3) | 0 (0) | 37 (23) | 118 (74) | 160 | 1.00 (NA)b | 0.76 (0.69–0.83) | 0.17 (0.04–0.30) |
T. pyogenes | 21 (13) | 8 (5) | 1 (1) | 125 (81) | 155 | 0.72 (0.53–0.87) | 0.99 (0.96–0.99) | 0.79 (0.65–0.92) |