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Table 1 List of 118 bacterial isolates and results of the RespoCheck bacterial multiplex real-time PCR

From: Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid

Identificationa(number of isolates tested)

CCUG identificationb

Source

RespoCheck bacterial multiplex real-time PCR(Ct values)

P. multocida

M. haemolytica

H. somni

T. pyogenes

Acidovorax spp. (n = 3)

NAc

WBVR collectiond

- g

-

-

-

Actinomyces spp

NA

WBVR collectiond

-

-

-

-

Aerococcus viridans

NA

WBVR collectione

-

-

-

-

Biberstenia trehalosi

NA

WBVR collectiond

-

-

-

-

Brucella abortus

NA

WBVR collectione

-

-

-

-

Comamonas kerstersii

NA

WBVR collectiond

-

-

-

-

Corynebacterium bovis (n = 2)

NA

WBVR collectione

-

-

-

-

Corynebacterium pseudotuberculosis

NA

WBVR collectione

-

-

-

-

Escherichia coli

NA

WBVR collectione

-

-

-

-

Gallibacterium anatis (n = 5)

NA

WBVR collectiond

-

-

-

-

Hafnia alvei

NA

WBVR collectiond

-

-

-

-

Histophilus somni

NA

ATCC 22132f

-

-

15.2

-

Histophilus somni (n = 4)

NA

WBVR collectiond

-

-

12.3–14.1

-

Klebsiella oxytoca

NA

WBVR collectione

-

-

-

-

Klebsiella pneumoniae

NA

WBVR collectione

-

-

-

-

Lactobacillus mucosae

NA

WBVR collectiond

-

-

-

-

Lactococcus garvieae

NA

WBVR collectione

-

-

-

-

Lactococcus lactis

NA

WBVR collectione

-

-

-

-

Listeria monocytogenes

NA

WBVR collectione

-

-

-

-

Mannheimia haemolytica

NA

ATCC 14003f

-

16.7

-

-

Mannheimia haemolytica (n = 5)

NA

WBVR collectiond

-

13.0–14.0

-

-

Mannheimia haemolytica

Mannheimia ruminalis

CCUG 38470-T

-

15.5

-

-

Mannheimia haemolytica

Mannheimia glucosida

CCUG 38457-T

-

16.0

-

-

Mannheimia granulomatis

Mannheimia granulomatis

CCUG 45422-T

-

-

-

-

Mannheimia varigena

Mannheimia varigena

CCUG 38462-T

-

-

-

-

Micrococcus luteus

NA

WBVR collectione

-

-

-

-

Moraxella bovis

NA

WBVR collectione

-

-

-

-

Moraxelle lacunata (n = 2)

NA

WBVR collectiond

-

-

-

-

Pantoea agglomerans (n = 13)

NA

WBVR collectiond

-

-

-

-

Pasteurella multocida

NA

ATCC 15743f

21.5

-

-

-

Pasteurella multocida (n = 23)

NA

WBVR collectiond

15–17

-

-

-

Pasteurella multocida

Bisgaard Taxon 13

CCUG 16497

17.9

-

-

-

Pasteurella multocida

Bisgaard Taxon 13

CCUG 16498

17.6

-

-

-

Pasteurella multocida

Pasteurella multocida subsp. gallicida

CCUG 17978-T

18.0

-

-

-

Pasteurella multocida

Pasteurella multocida subsp. septica

CCUG 17977-T

17.0

-

-

-

Not typable

Pasteurella aerogenes

CCUG 27905

-

-

-

-

Proteus mirabillis

NA

WBVR collectiond

-

-

-

-

Pseudomonas aeruginosa

NA

WBVR collectione

-

-

-

-

Psychrobacter spp.

NA

WBVR collectiond

-

-

-

-

Salmonella enterica subsp. enterica serovar Dublin

NA

WBVR collectione

-

-

-

-

Salmonella enterica subsp. enterica serovar Typhimurium

NA

WBVR collectione

-

-

-

-

Serratia marcescans

NA

WBVR collectione

-

-

-

-

Staphylococcus aureus

NA

WBVR collectione

-

-

-

-

Staphylococcus epidermidis

NA

WBVR collectione

-

-

-

-

Streptococcus agalactiae

NA

WBVR collectione

-

-

-

-

Streptococcus bovis (n = 5)

NA

WBVR collectiond

-

-

-

-

Streptococcus dysgalactiae

NA

WBVR collectione

-

-

-

-

Streptococcus faecalis

NA

WBVR collectione

-

-

-

-

Streptococcus pluranimalium (n = 5)

NA

WBVR collectiond

-

-

-

-

Streptococcus pneumoniae

NA

WBVR collectione

-

-

-

-

Streptococcus spp. (n = 3)

NA

WBVR collectiond

-

-

-

-

Streptococcus uberis

NA

WBVR collectione

-

-

-

-

Trueperella pyogenes

NA

ATCC 9731f

-

-

-

15.8

Trueperella pyogenes (n = 5)

NA

WBVR collectiond

-

-

-

12.8–16.0

Yersinia enterolytica

NA

WBVR collectione

-

-

-

-

  1. aIsolates were identified with MALDI-TOF mass spectrometry (as described in Materials and Methods)
  2. bCCUG: Culture Collection University of Gothenburg (Sweden)
  3. cNA: Not applicable
  4. dIsolated by Wageningen Bioveterinary Research (WBVR) from lungs of calves in the course of an earlier field study to BRD
  5. eCattle isolates from tissue other than lung
  6. fATCC: American Type Culture Collection (USA)
  7. g-: Ct value above 40