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Table 3 Effect of the selection with different concentrations of Androcoll-Bear on sperm membrane and acrosomal status

From: Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm

TIME

TREATMENT

%Viab

%Apoptosis

%Viable_Acro

0 h

Control

46.7 ± 4 A

24.2 ± 4.3 A

75.4 ± 3.6 A

 

A50

58.4 ± 2.7 A

16 ± 2.4 A

81.6 ± 2.1 A

 

A65

73.9 ± 2.4 *;A

15 ± 2.8 A

88 ± 1.4 *;A

 

A80

78.1 ± 2.3 *;A

15.3 ± 2.6 A

93 ± 1.2 *;A

2 h

Control

31.7 ± 3.9 B

24.1 ± 5.5 A

55.9 ± 5.2 B

 

A50

45.6 ± 3.6 B

16.9 ± 1.9 A

64.1 ± 4 B

 

A65

50.7 ± 5.1 *;B

16.8 ± 1.7 A

68.4 ± 4.3 B

 

A80

56.1 ± 4.2 *;B

19.6 ± 2 A

75.9 ± 4.2 *;B

  1. The table shows: viability (%Viab), apoptosis (%Apoptosis) and viable sperm with intact acrosome (%Viable_Acro). Time: immediately after thawing (0 h) and after an incubation of 2 h at 37 °C (2 h). Treatments: Androcoll-Bear 50% (A50), Androcoll-Bear 65% (A65) and Androcoll-Bear 80% (A80). Data shown are the model-derived mean ± s.e.m
  2. (*) Asterisks show differences (P < 0.05) between a specific Androcoll-Bear proportion and its Control
  3. (A,B) Capital letters show differences (P < 0.05) for the same treatment between time (0 and 2 h)
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BMC Veterinary Research

ISSN: 1746-6148

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