Table 3 Primers for detection of mollicutes
PCR assay | Identification | Sequence (5′-3′) | Amplicon size (bp) | Gene target | Reference |
|---|---|---|---|---|---|
1 | Myc23F1729 | 5′ CTAAGGTDAGCGAGWDAACTAT AG 3’ | 102–110 | 23S rRNA | Hotzel et al. [17] |
Myc23R1843 | 5′ CCCCYCWTSYTTYACTGMGGC 3’ | ||||
2 | MW28 | 5′ CCAGACTCCTACGGGAGGCA 3’ | 580 | 16S rRNA | Spergser et al. [27] |
MW29 | 5′ TGCGAGCATACTACTCAGGC 3’ | ||||
3 | GC341F | 5’CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGCCTACGGGAGGCAGCAG 3’ | 340 | 16S rRNA | McAuliffe et al. [18] |
R543 | 5′ ACCTATGTATTACCGCG 3’ | ||||
4 | GPO3 | 5′ GGGAGCAAACAGGATTAGATAC CCT 3’ | 270 | 16S rRNA | Van Kuppeveld et al. [6] |
MGSO | 5′ TGCACCATCTGTCACTCTGTTAACCTC 3’ | ||||
5 | GPO3F | 5′ TGGGGAGCAAACAGGATTAGAT ACC 3’ | 270 | 16S rRNA | Botes et al. [28] |
MGSO | 5′ TGCACCATCTGTCACTCTGTTAACCTC 3’ | ||||
6 | 16S + C | 5′ CGTTCTCGGGTCTTGTACAC 3’ | 400–600 | ISRa | Ramírez et al. [16] |
23S–B | 5′ CGCAGGTTTGCACGTCCTTCATCG 3’ |