Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes

Vega-Orellana, O.; Poveda, J. B.; Rosales, R. S.; Bradbury, J. M.; Poveda, C. G.; Mederos-Iriarte, L. E.; Tavío, M. M.; Ramírez, A. S.

doi:10.1186/s12917-017-1116-2

BMC Veterinary Research

Table 1 Results of six different PCR assays using a mollicutes DNA panel

From: Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes

Mollicutes species	Strain	PCR assays^a						Real-time PCR^b melting Temperature (°C)
Mollicutes species	Strain	1	2	3	4	5	6	Real-time PCR^b melting Temperature (°C)
Acholeplasma (A.) granularum	BTS-39	−	+	+	+	+	+/−	86.5
A. laidlawii	PG8	−	+	+	+	+	+/−	86.5
A. modicum	PG49	−	+	+	+	+	+/−	86.5
A. oculi	19-L	−	+	+	+	+	+/−	85
A. parvum	H23M	−	+	+	+	+	+/−	85
Mesoplasma florum	L1	−	+	+	+	+	+	86
Mycoplasma (M.) agalactiae	PG2	+	+	+	+	+	+	85.5
M. alkalescens	PG51	+	+	+	+	+	+	86
M. arginini	G230	+	+	+	+	+	+	86
M. auris	CIP 105677	+	+	+	+	+	+	86
M. bovigenitalium	PG11	−	+	+	+	+	+	86
M. bovirhinis	PG43	+	+	+	+	+	+	87
M. bovis	PG45	−	+	+	+	+	+	86
M. bovoculi	M165/69	−	+	+	+	+	+	85.5
M. canadense	275C	−	+	−	+	+	+	86
M. capricolum subsp. capricolum	California Kid	+	+	+	+	+	+	87
M. capricolum subsp. capripneumoniae	F38	−	+	+	+	+	+	86.5
M. caviae	G122	−	+	+	+	+	+	86
M. columbinum	MMP-1	+	+	+	+	+	+	85.5
M. columbinasale	694	+	+	+	+	+	+	85.5
M. columborale	MMP-4	+	+	+	+	+	+	86.5
M. cottewii	VIS	+	+	+	+	+	+	87
M. fermentans	A918 C1	+	+	+	+	+	+	86
M. flocculare	Ms42	−	+	+	+	+	+	86.5
M. gallinaceum	DD	−	+	+	+	+	+	86
M. gallinarum	PG16	−	+	+	+	+	+	86
M. gallisepticum	PG31	+	+	+	+	+	+	86
M. gallopavonis	WP1	−	+	−	−	−	+	86.5
M. hominis	PG21	+	+	+	+	+	+	86.5
M. hyopharyngis	H3-6B	+	+	+	+	+	+	85.5
M. hyopneumoniae	Ms42	−	+	+	+	+	+	86.5
M. hyorhinis	BTS-7	−	+	+	+	+	+	86.5
M. hyosynoviae	S16	−	+	+	+	+	+	86
M. imitans	4229	+	+	+	+	+	+	86.5
M. iners	PG30	+	+	+	+	+	+	85.5
M. iowae	695	+	+	+	+	+	+	85
M. leachii	PG 50	+	+	+	+	+	+	86
M. lipofaciens	ML64	+	+	+	+	+	+	86
M. maculosum	PG 15	−	+	+	+	+	+	86
M. meleagridis	17,529	−	+	+	+	+	+	86
M. mycoides subsp. capri	Y-GOAT	+	+	+	+	+	+	87
M. mycoides subsp. mycoides SC	PG1	+	+	+	+	+	+	87
M. neophronis	G.A.	+	+	+	+	+	+	86
M. opalescens	MH5408	+	+	+	+	+	+	86
M. phocicerebrale	D’049	+	+	+	+	+	+	86
M. phocidae	105	+	+	+	+	+	+	86
M. phocirhinis	DG52	+	+	+	+	+	+	86
M. sp. Phocoena	C-269	+	+	+	+	+	+	86.5
M. pneumoniae	FH M III	+	+	+	+	+	+	86
M. pullorum	CKK	+	+	+	+	+	+	86
M. putrefaciens	KS1	+	+	+	+	+	+	87
M. spumans	PG13	+	+	+	+	+	+	86
M. sualvi	Mayfield B	+	+	+	+	+	+	86
M. synoviae	WVU 1853	−	+	+	+	+	+	84.5
M. verecundum	107	−	+	+	+	+	+	86
M. wenyonii ^c	Massachusetts	+	+	+	+	+	+	84.5
M. yeatsii	GIH	+	+	+	+	+	+	87
M. zalophi	CSL 4296	+	+	+	+	+	+	86
M. zalophidermidis	4779	+	+	+	+	+	+	86
Phytoplasma	PD	NT^d	NT	NT	NT	NT	NT	84.5^e
Phytoplasma	ESFY	NT	NT	NT	NT	NT	NT	84.5^e
Spiroplasma spp.	−	+	+	+	+	+	+	87
Ureaplasma urealyticum	960	+	+	+	+	+	+	85

^aPCR assays: 1, Hotzel et al. [17]; 2, Spergser et al. [27], 3, McAuliffe et al. [18]; 4, Van Kuppeveld et al. [6]; 5, Botes et al. [28]; 6, Ramírez et al. [16] and real-time PCR application of PCR assays n°4 and n°5
^bReal-time PCR was performed with primers pairs number 4 and 5, giving both the same result
^cDNA extracted, identified and donated by Animal and Plant Health Agency (APHA, UK)
+: amplification test positive -: amplification test negative ±: amplification of more than one DNA fragment
^dNT: Not tested
^ePhytoplasma DNA extracted, identified and donated by Assunta Bertaccini (University of Bologna) was only tested using Botes et al. [28] real-time assay

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