Fig. 1From: Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes Melting curves for PCR products for the specific detection of mollicutes obtained using Botes et al. assay. Positive samples produced a melting peak between 84 and 87 °C. Negative samples did not produce any melting peak. Unspecific reactions produced a melting peak between 82 and 83 °C. Mollicutes DNA control: M. hyorhinis. Non mollicutes DNA control: Staphylococcus aureus Back to article page