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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Evaluation of P16 expression in canine appendicular osteosarcoma

Fig. 3

Immunohistochemistry assays reveal the proportion of neoplastic cells expressing P16. Canine glioblastoma cells demonstrate abundant red-brown staining in both the cytoplasm and nucleus (GBM, + control P16 IHC) (a). Normal canine brain tissue demonstrates an absence of red-brown stained cells (NB, negative control P16 IHC) (b). In a canine OSA, a majority of the neoplastic cells demonstrate red-brown cytoplasmic staining (3+ staining, case 19) (c). In a canine OSA, approximately 50% of the neoplastic cells demonstrate red-brown cytoplasmic staining (2+ staining, case 9) (d). In canine OSA, less than 25% of the neoplastic cells demonstrate red-brown cytoplasmic staining (1+ staining, case 4) (e). In canine OSA, none of the neoplastic cells demonstrate red-brown cytoplasmic staining (0+ staining, case 13). P16 immunohistochemistry, original magnification 400×

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